Analysis of chicken macrophage functions and gene expressions following infectious bronchitis virus M41 infection
نویسندگان
چکیده
منابع مشابه
Molecular Cloning and Expression of Nucleocapsid Gene of Chicken Infectious Bronchitis Virus Strain Massachusetts H120
Chicken infectious bronchitis virus (IBV) causes severe respiratory and renal dysfunction in chickens. In the present study, we used reverse transcription-polymerase chain reaction (RT-PCR) to amplify the nucleocapsid (N) protein gene of strain Massachusetts (Mass) H120 of IBV that is commonly used in the vaccine production in Iran. The PCR product with the expected size of 1.2 kb was cloned in...
متن کاملInfectious Bronchitis Virus Infection Induces Apoptosis during Replication in Chicken Macrophage HD11 Cells
Avian infectious bronchitis has caused huge economic losses in the poultry industry. Previous studies have reported that infectious bronchitis virus (IBV) infection can produce cytopathic effects (CPE) and apoptosis in some mammalian cells and primary cells. However, there is little research on IBV-induced immune cell apoptosis. In this study, chicken macrophage HD11 cells were established as a...
متن کاملSequences and Phylogenetic Analysis of Envelope (E) Gene of Iranian Infectious Bronchitis Virus Isolates in Iran
Background and Aims: Infectious bronchitis virus (IBV) is the etiological agent of infectious bronchitis (IB), which is an acute and highly contagious disease of the respiratory and sometimes the urogenital tracts of chickens. In Iran, the disease has been identified in chicken flocks with serological and virus isolation methods. Gene 3c (E) encode envelop (small membrane) protein. For envelope...
متن کاملmolecular cloning and expression of nucleocapsid gene of chicken infectious bronchitis virus strain massachusetts h120
chicken infectious bronchitis virus (ibv) causes severe respiratory and renal dysfunction in chickens. in the present study, we used reverse transcription-polymerase chain reaction (rt-pcr) to amplify the nucleocapsid (n) protein gene of strain massachusetts (mass) h120 of ibv that is commonly used in the vaccine production in iran. the pcr product with the expected size of 1.2 kb was cloned in...
متن کاملPrevalence of avian infectious bronchitis virus in broiler chicken farms in south of Iraq, 2014 – 2015
Avian infectious bronchitis (IB), caused by a gammacoronavirus, is an OIE-listed (List B) disease and characterized by respiratory and renal involvements, causing high mortality, and economic loss in both layers and broilers. In comparison with other diagnostic methods, real-time polymerase chain reaction (RT-PCR) and conventional RT-PCR are potent, more sensitive and faster techniques for infe...
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ژورنال
عنوان ژورنال: Veterinary Research
سال: 2021
ISSN: 1297-9716
DOI: 10.1186/s13567-021-00896-z